Introduction

The Meta Overview combines a sample profiling barplot, an abundance distribution heatmap, a significant differential taxonomy box plot, and a metadata heatmap into one diagram. This combination clearly displays the correlation between taxonomy and metadata. Samples can be re-ordered by metadata to detect patterns.

If you have the raw profiling table of samples without differential testing analysis, you can use Overview module.

If you want to visualize your processed results, you can upload the following inputs to visualize online interactively:

Input Files

Click on the Download Data button to check the official input files.

1. Profiling tables (TSV file)

The profiling table you want to visualize should contain the samples exactly the same in the metadata file. The abundance of each sample should sum to 100. We recommend you not contain more than 20 taxonomy in the input. If you use the raw abundance of all taxonomy levels, we recommend you use the (Module Overview)[https://gutmeta.deepomics.org/submit/analysis/meta-overview] will filter the top 20 genus as input.

2. Metadata (TSV file)

• header example
  

Here is an example of the header of the metadata file. The attribute will be used in the visualization for color, shape, and sider boxplot. The metadata can be continuous (e.g., Age) and discrete (e.g., gender) variables. The first column should be the sample name.

• rows
  sample ID and the metadata for the corresponding sample.

Display Interactions

• Highlight
  Hover on the main matrix grid and the rest relative chart will be highlighted by bounding black rect.
• Tooltips
  Hover on the box and the tooltip of data will show.
• Drag
  Drag a legend to change its position.
• Download
  One SVG file will be generated when the 'Download' button is clicked.

Sidebar Functions

• Files

  • Manage Files: checklist of files uploaded previously, delete or download files.
  • Upload: upload files. Note that the duplicated file name will be alerted and given a random postfix.
  • Choose: choose files uploaded previously. > Notice that those input data accepting multiple files use all uploaded files, so you cannot choose specific files for those data.

• Data

  • Taxonomic Rank: select the rank of the data to display.
  • Range lower bound: edit the mininum of the value range.
  • Range upper bound: edit the maximum of the value range.

• Plot Setting
  

  • Rotate x labels: display rotated labels aligned middle-right or normal labels aligned top-centre

Manual version=1.1, updated by Dr. JIANG Yiqi on 2022-04-19.

Manual version=1.0, written by Dr. JIANG Yiqi and Ms. WANG Yanfei on 2021-09-13.